There are 24 Golden Bugs, male and females of the same species, scattered across the wilds in Zelda: Twilight Princess. Agitha, located at the eastern tip of the Castle Town, will ask you to collect them.

The first Bug nets you the Big Wallet, which moves your max number of rupees up to 600. Every bug after that nets you 50 rupees; if you manage to complete a set, two bugs of the same species of the opposite sex, she will grant you 100 rupees.

Providing her with all 24 of the bugs nets you the Giant Wallet, which moves your max number of rupees out to 1000.

Best advice I can give about finding these bugs is to hunt them at night. They are very easy to see then, as they glow in the dark. Being in wolf form makes this an even easier task to accomplish. The game even announces it to you, providing a distinct metallic chime when you’re nearby.

However, since you’re here, I’m assuming that you want to know their locations anyway, hmm?

Male Bugs:

Ant: Male. By the northwestern tree in the graveyard.

Beetle: Male. In Hyrule Field. Head south east from the bridge. It will be high up on the side of a lone tree.

Butterfly: Male. Around the flowers to the southeast of the east gate of Castle Town.

Dayfly: Male. Head west from the Cave of Ordeals until you see it.

Dragonfly: Male. West from the waterfall in Zora’s Domain and up the hill. It will be nearby some crates.

Grasshopper: Male. In the field north of Kakariko Village. In the south eastern area, on the ground.

Ladybug: Male. To the south and west of Castle Town, where the blockage is. It is up on the wall.

Mantis: Male. Go to the north end of the bridge across Lake Hylia. Look between its pillars, it will be flying around there.

Phasmid: Male. Southern end of Eldin Bridge, on the side of the nearby pillar.

Pill Bug: Male. Head to the southern end of the Bridge over Kakariko Gorge. The bug is nearby the cliff edge to the fence.

Snail: Male. Go to the Sacred Grove. Move the block and go down into the cave. The bug will be on a wall.

Stag Beetle: Male. Head east from the river at the north end of Hyrule field till you come across a lone tree; the bug will be there.

Female Bugs:

Ant: Female. In the westernmost house in Kakariko Village.

Beetle: Female. Head to the high ledge in the west of Hyrule Field. Follow it, checking the trees you come to; the bug will be on one of these trees. Use the Clawshot or Boomerang to snag it.

Butterfly: Female. Head East from Castle Town until you come across a high ledge. Clawshot up there and nab the bug.

Dayfly: Female. North of where the chest, resting atop a pillar is in the southeast of the desert. The insect is in one of the trenches.

Dragonfly: Female. Floating over the water in front of the Boat Shop on Zora’s River. Clawshot or snag it with the Boomerang.

Grasshopper: Female. In the field north of Kakariko Village. In the northeast section this time.

Ladybug: Female. South of Castle Town, in the fields, go east from the pool in the courtyard. The ladybug is in one of the three trees standing by themselves.

Mantis: Female. The rocky overpass south of the bridge across Lake Hylia. It will be on the wall near the overpass. Use the Clawshot or the Boomerang to snag it.

Phasmid: Female. Northern end of the Eldin Bridge. Find the cliff to the north and Clawshot up there.

Pill Bug: Female. Check the flowers to the south of the southern entrance to Kakariko Village.

Snail: Female. Go to the Temple of Time. Down the stairs and to the left of the steps. Use the Clawshot or Boomerang to snag it.

Stag Beetle: Female. Go to the path leading into the mountains. Head right where the path splits, until you come to a boulder in the wall. The bug will be above the boulder. Use the Clawshot or the Boomerang to snag it.