As an aspiring 00 agent dismissed from MI6 for reckless brutality, the player is recruited by Auric Goldfinger in a ruthless war against Dr. No for control of the Bond underworld. Players wreak havoc as they make their unrelenting rise through the ranks of villainy. On globe-spanning missions of vengeance and demolition, players encounter such legendary Bond characters as Oddjob, Dr. No, Goldfinger, Scaramanga, and Xenia Onatopp.

Gamers outwit villainous rivals and battle it out in the intense wireless multiplayer mayhem with up to seven other players using only one cartridge, a unique feature for the Nintendo DS. The game also features a robust single-player campaign divided into six missions. Players can exploit multiple weapon combinations through the dual-wield gunplay system, or go head-to-head, melee-style, by taking enemies hostage or knocking them out with one punch.